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Part:BBa_K1463031:Design

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-09-09)


Prefix and extra nucleotides for switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 31
    Illegal XbaI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
    Illegal NotI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
    Illegal BglII site found at 25
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 31
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 31
    Illegal XbaI site found at 5
    Illegal XbaI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Switch required extra nucleotides and restriction site added to check function of switch.


Source

Synthesised by IDT from a given sequence.

References